Chek1 Polyclonal Antibody for Mouse(Mus musculus) | EIAab.comChek1 Polyclonal Antibody for Mouse(Mus musculus)

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Chek1 (Gene name),

Serine/threonine-protein kinase Chk1 (Protein name ), CHK1_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

Chek1(Chk1);

Protein name:

Serine/threonine-protein kinase Chk1;

Alternative:

Checkpoint kinase-1;CHK1 checkpoint homolog;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Interacts with BRCA1, CLSPN, FBXO6, PPM1D, RAD51, TIMELESS, XPO1/CRM1 and YWHAZ/14-3-3 zeta (By similarity). Interacts with FEM1B.

Function:

Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-171' and 'Thr-497' and phosphorylation of CDC25C creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-74', 'Ser-122', 'Ser-171', 'Ser-271' and 'Ser-284' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation. Phosphorylates NEK6 and NEK11. Phosphorylation of CDC25A at Ser-74 primes it for subsequent phosphorylation at Ser-77, Ser-80 and Ser-86 by NEK11 (By similarity). Essential for early embryogenesis.

Subcellular Location:

Nucleus Cytoplasm Cytoplasm cytoskeleton centrosome Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B.

Protein Attributes:

Sequence length:

476

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

476:

FKRHFLKIKG | KLSDVVSSQK | VWFPVT

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

mmu:12649

Pfam:

PF00069

SMR:

O35280

UniGene:

Mm.16753

String:

O35280

Uniprot:

O35280

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ELISA Kit for Mouse Serine/threonine-protein kinase Chk1

Cat.:

E11379m

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96T

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ELISA Kit for Mouse Serine/threonine-protein kinase Chk1

Cat.:

E11379c

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96T

ELISA Kit for Mouse Serine/threonine-protein kinase Chk1

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E11379r

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ELISA Kit for Mouse Serine/threonine-protein kinase Chk1

Cat.:

E11379h

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96T

CLIA Kit for Mouse Serine/threonine-protein kinase Chk1

Cat.:

U11379r

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96T

CLIA Kit for Mouse Serine/threonine-protein kinase Chk1

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U11379h

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96T

CLIA Kit for Mouse Serine/threonine-protein kinase Chk1

Cat.:

U11379c

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96T

CLIA Kit for Mouse Serine/threonine-protein kinase Chk1

Cat.:

U11379m

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96T

Polyclonal Antibody for Mouse Serine/threonine-protein kinase Chk1

Cat.:

P11379Rb-h

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40ug/0.2ml

Polyclonal Antibody for Mouse Serine/threonine-protein kinase Chk1

Cat.:

P11379Rb-r

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Polyclonal Antibody for Mouse Serine/threonine-protein kinase Chk1

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P11379Rb-m

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Polyclonal Antibody for Mouse Serine/threonine-protein kinase Chk1

Monoclonal Antibody for Mouse Serine/threonine-protein kinase Chk1

Protein for Mouse Serine/threonine-protein kinase Chk1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25."
Sanchez Y. , Wong C. , Thoma R.S. , Richman R. , Wu Z. , Piwnica-Worms H. , Elledge S.J.
Science277:1497-1501(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);TISSUE SPECIFICITY
2.

"The transcriptional landscape of the mammalian genome."
Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
strain: C57BL/6J.
tissue: Embryo.
tissue: Testis.
3.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
tissue: Mammary gland.
4.

"Atm-dependent interactions of a mammalian chk1 homolog with meiotic chromosomes."
Flaggs G. , Plug A.W. , Dunks K.M. , Mundt K.E. , Ford J.C. , Quiggle M.R.E. , Taylor E.M. , Westphal C.H. , Ashley T. , Hoekstra M.F. , Carr A.M.
Curr. Biol.7:977-986(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 204-450 (ISOFORMS 1/2);SUBCELLULAR LOCATION;DEVELOPMENTAL STAGE
5.

"Aberrant cell cycle checkpoint function and early embryonic death in Chk1(-/-) mice."
Takai H. , Tominaga K. , Motoyama N. , Minamishima Y.A. , Nagahama H. , Tsukiyama T. , Ikeda K. , Nakayama K. , Nakanishi M. , Nakayama K.
Genes Dev.14:1439-1447(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;DISRUPTION PHENOTYPE
6.

"Chk1 is an essential kinase that is regulated by Atr and required for the G(2)/M DNA damage checkpoint."
Liu Q. , Guntuku S. , Cui X.-S. , Matsuoka S. , Cortez D. , Tamai K. , Luo G. , Carattini-Rivera S. , DeMayo F. , Bradley A. , Donehower L.A. , Elledge S.J.
Genes Dev.14:1448-1459(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN G2/M DNA DAMAGE CHECKPOINT
7.

"Chk1 is haploinsufficient for multiple functions critical to tumor suppression."
Lam M.H. , Liu Q. , Elledge S.J. , Rosen J.M.
Cancer Cell6:45-59(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
8.

"Lack of PTEN sequesters CHK1 and initiates genetic instability."
Puc J. , Keniry M. , Li H.S. , Pandita T.K. , Choudhury A.D. , Memeo L. , Mansukhani M. , Murty V.V.V.S. , Gaciong Z. , Meek S.E.M. , Piwnica-Worms H. , Hibshoosh H. , Parsons R.
Cancer Cell7:193-204(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;UBIQUITINATION;PHOSPHORYLATION AT SER-280 AND SER-345;MUTAGENESIS OF SER-280
9.

"Mice lacking protein phosphatase 5 are defective in ataxia telangiectasia mutated (ATM)-mediated cell cycle arrest."
Yong W. , Bao S. , Chen H. , Li D. , Sanchez E.R. , Shou W.
J. Biol. Chem.282:14690-14694(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-317
10.

"Chk1 is a histone H3 threonine 11 kinase that regulates DNA damage-induced transcriptional repression."
Shimada M. , Niida H. , Zineldeen D.H. , Tagami H. , Tanaka M. , Saito H. , Nakanishi M.
Cell132:221-232(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN EPIGENETIC REGULATION OF TRANSCRIPTION
11.

"Mammalian TIMELESS is involved in period determination and DNA damage-dependent phase advancing of the circadian clock."
Engelen E. , Janssens R.C. , Yagita K. , Smits V.A. , van der Horst G.T. , Tamanini F.
PLoS ONE8:E56623-E56623(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TIMELESS

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